Domains are distinct functional and structural units that are often responsible for a particular function or interaction, which define the proteins overall role [1]. Proteins can contain multiple domains with unique functions, such as catalyzing a specific pathway or binding a specific molecule. Domains are also evolutionary conserved portions of a protein sequence that can evolve, function, and exist independently of the rest of the protein.
What are the domains of CLCN1
Online programs SMART, Pfam and InterPRo were used to search the Human CLCN1 protein for domains. The results are displayed below:
Results given from SMART
results given from Pfam
Results of InterPRo
Both Pfam and InterPro identified one large domain within CLCN1, while SMART identified mutiple domains.
How conserved are the domains of CLCN1?
Disscusion
As seen above, the chloride channel protein domains are highly conserved among most of the selected organisms. Interestingly, Zebra fish, chicken and mouse have an extra CBS binding domain. Protein domains are important because they are used to analysis important information about protein function within its sequence. Mutations within domains are likely to cause a corresponding loss of function, it is useful when considering the effects of mutation.